Sensitive skin, skin ageing, wound healing, psoriasis, eczema… are pathologies involving both the skin and neurons. Cosmetic developments are limited by current models, either because they use animal neurons or because they do not reproduce physiology. Indeed, while animal experimentation is prohibited in the cosmetic field, preclinical models are especially limited to evaluate the relationship between neurons and epithelium such as the skin. In fact, anatomically, the neuronal cell bodies are located in the spinal cord and are connected to the skin by their axonal projections. Therefore, mixing neurons and epithelial cells in the same compartment is not representative of the real physiology, limits the use of specific culture media and biases the evaluation of the effect of an ingredient. A relevant compartmentalized and fully human preclinical model could then better predict outcome.

We have developed at NETRI a compartmentalized microfluidic device to co-culture of hiPSC-derived sensory neurons with primary keratinocytes to ensure both fluidic isolation and physical connexion between axons and skin cells and thus to mimic the anatomical structure.

CAPABILITIES Co-culture, hiPSC Derived Cell, Readouts
CHIP DuaLink
CELL TYPE Sensory Neurons & Primary Keratinocytes
RESOURCES Publications, Application Note, Application Protocol, Posters, Cells DataSheet
(Axol Bioscience)
READINESS LEVEL ④/ ⑧ Protocol done for sensory neurons