Starter OoC Kits.

Specially designed for kick start microfluidics and electrophysiology recording experience:
• 4 QuarterBentos of DuaLink MEA
• 2 NeoBentos MEA EDGE frame with 4 plugs
• UpLink utility software
• Online training on microfluidics and electrophysiology recording & 1-hour of support meeting

What do you need to use it?
• Maestro EDGE or PRO, powered by Axion BioSystems
• UpLink downloadable from NETRI Support Page
• Standard equipments (biological safety cabinet, micropipettes etc…)
• Standard consumables (Ethanol 70%, distilled water etc…)
• Your cells and coating & media solutions associated
To see all equipment and consumables required, please refer to our DuaLink MEA Protocol.

All our chips are in the NeoBento™ MEA format (standard 96-well SBS plate format) and thus offer a variety of handling readouts.

Electrophysiology (Using AxIS and NETRI’s Uplink utility software
• Axion MEA metrics (per channel / µchannel)
• Axion MEA Network Metrics (per channel)
• Individual Axion Electrode Metrics
• Compartimentalized Rasterplots

Imaging
• Immunofluorescence
• Calcium Imaging
• Live staining
• Live Dead Assays

Biochimic analysis
• Lysis cells analysis
• Supernatant analysis
• ELISA
• Liquid chromatography
• Mass Spectroscopy

In-vitro models:
• Nociceptive Pain
• Innervated skin 2D

Applications
• Study of the functional activity of neurons
• Analysis of the functional influence of a non-neuronal cell population on neurons
• Axonal transport
• Skin nociception
• Itch
• Ageing
• Wound healing
• Toxicology
• Virology (viral transfection in one compartment only)
• Neuroinflammation (Multiple sclerosis, Cerebral tumors…)
• Innervated skin
• Neuromuscular junction
• Motor neuron diseases (Amyotrophic Lateral Sclerosis…)

Our aim is to translate physiological behaviors by integrating our technologies within one chip.

• Electrophysiology: Combining our advanced organs-on-chip devices with Axion Biosystems’ Maestro MEA benchtop systems allows users to record and analyze each compartmentalized cell population independently, allowing them to better understand the relationships and effects at play.

• Microchannel tunnels: Due to their micrometric measurements, only cell extensions can grow within the microchannels, leaving the cell bodies within the compartments themselves, that allow an enhanced fluidic isolation and discontinuous connectivity between compartments.

• NeoBento HT Format: The NeoBento is our versatile container which is in the standard 96-well microplate format, compatible with standard liquid handling and imaging equipment, and does not require expensive equipment. The NeoBento™ makes NETRI’s microfluidic devices as versatile and easy to handle as a typical 96-well plate.