Parkinson’s disease (PD) is a neurodegenerative disease that causes involuntary or uncontrollable movements. The most prominent signs and symptoms of PD occur when dopaminergic neurons in the basal ganglia degenerate. MPTP and 6OHDA are 2 neurotoxins involved in the aggregation of alpha-synuclein (α-Syn) peptide and in the degeneration of dopaminergic neurons. Currently, the internalization of these 2 toxins in neurons is still an unknown mechanism as well as their actions on the α-Syn peptide.
We have developed at NETRI compartmentalized microfluidic devices with an enhanced fluidic isolation allowing neurotoxins testing only on somas or axons.
|CAPABILITIES||Co-culture, hiPSC Derived Cell, Readouts|
|CELL TYPE||Dopaminergic Neurons|
|RESOURCES||Publications, Application Note, Application Protocol, Posters, Cells DataSheet|
|(FujiFilm CDI, BrainXell), Chip DataSheet|
|RELATED PAPERS||Seidi, A., Kaji, H., Annabi, N., Ostrovidov, S., Ramalingam, M., & Khademhosseini, A. (2011). A microfluidic-based neurotoxin concentration gradient for the generation of an in vitro model of Parkinson’s disease. Biomicrofluidics, 5(2), 1–14.|
|READINESS LEVEL||④/ ⑧ Protocol done|