3D ECM EMBEDDED ORGANOIDS.
3D LIVER ORGANOIDS.
PRIMARY HUMAN HEPATOCYTES.
Description.
Liver organoids grown in BioMIMESYS® Liver faithfully reproduce the key functions of the human liver: +20 to 30x albumin secretion compared with 2D cultures, high expression of CYP450 enzymes (CYP3A4, CYP1A1/2, CYP2B6), formation of a network of canaliculi (Actin/MRP2) and response to treatment (e.g. lipid accumulation with amiodarone).
Readouts.
• Teer compatibility.
• Imaging.
Advantages.
• Enhance specific hepatic gene expression and metabolism gene expression.
• Increase cell survival and enabled high dose testing.
Potential Applications.
• Toxicology screening.
• Metabolism and liver diseases (NASH, steatosis, fibrosis).
3D PANCREATIC ORGANOIDS.
PANC-1 CELL LINE.
Description.
PANC-1 cells grown in BioMIMESYS® form growing, homogeneous spheroids in BioMIMESYS®, providing a stable platform for: analysis of tumor growth, assessing the cytotoxicity of chemotherapies and the study of invasion and responses in the tumor microenvironment.
Readouts.
• Teer compatibility.
• Imaging.
Advantages.
• Homogeneous spheroids.
Potential Applications.
• Metabolic activity.
• Pancreatic oncology and 3D tumor modeling therapeutic response. growing, homogeneous spheroids
3D ADIPOSE TISSUE ORGANOIDS.
HUMAN PRIMARY PRE-ADIPOCYTES.
Description.
Human pre-adipocytes grown in BioMIMESYS® differentiate efficiently and retain their functionality for up to 2 months: adapted ECM architecture (HA + collagens I and VI), physiological elasticity (0.45 kPa) and compatible with primary cells and iPSCs.
Readouts.
• Teer compatibility.
• Imaging.
Advantages.
• Efficient differentiation and long-term culture.
• Maintain functionality for at least one month.
Potential Applications.
• Lipid metabolism screening.
• Obesity & nutritional screening.
• Diabetes screening.
3D BRAIN ORGANOIDS.
NEUROSPHERE & NEURONAL NETWORK.
Description.
Brain organoids grown in BioMIMESYS® form long-term functional 3D brain cultures, providing a stable platform for compounds screening.
Readouts.
• Teer compatibility.
• Imaging.
Advantages.
• Markers expression.
• Neurosphere and neuronal network.
Potential Applications.
• Parkinson's Diesease.
• Neurotoxicity.
3D TUMOROIDS.
CANCER CELLS & PRIMARY HUMAN MACROPHAGES.
Description.
BioMIMESYS® customizes the tumor microenvironment to better predict treatment efficacy. It is possible to modulate the rigidity (e.g. 1 kPa) and composition of the ECM matrix to simulate different tumor stages or types: measured impact on cell proliferation, marker monitoring (e.g. CD44, MDA-MB-231 spheroids, MCF7) and possibility of co-culturing with human immune cells.
Readouts.
• Teer compatibility.
• Imaging.
Advantages.
• Increase of cell proliferation.
• Markers expression.
Potential Applications.
• Immuno-oncology.
• Development of targeted therapies and resistance studies.
•Publication: In vitro three-dimensional cell cultures for bone sarcomas.
•Publication: A Promising Combination for Neuroendocrine Tumors Treatment.
•Publication: ABCA1/ABCB1 Ratio Determines Chemo- and Immune-Sensitivity in Human Osteosarcoma.
•Publication: Reticulated hyaluronan hydrogels: a model for examining cancer cell invasion in 3D.
OUR OFFERS.
Discovery Pack.
Familiarize yourself with NeoBentos MEA to initiate your compartmentalized electrophysiology model.
• 5 NeoBentos for 10 conditions and 8 replicates.
• UpLink Software.
• Online Training and 10 Hours of support meeting with our Field Application Scientist and/or scientists.
• Mono-culture SOP only (sensory neurons, motor neurons, keratinocytes, muscle etc...)
Development Pack.
Develop your own assay with NeoBentos MEA.
• Minimun 5 NeoBentos with volume discount (up to 35%).
• UpLink Cloud Software (secured cloud).
• On-site Training and 100 Hours of support meeting with our Field Application Scientist and/or scientists.
• All mono- and co-culture SOP (motor neurons, muscle, innervated skin, Gluta-GABA, etc...)
ARE YOU A PUBLIC RESEARCH LABORATORY?
At NETRI, academic research is a cornerstone of our mission. We firmly believe that major advances result from collaboration between public research laboratories and industry players. We offer products specially designed for academic teams to facilitate access to NAM industrial technologies.
