DERMATOLOGY PROGRAM.
INNERVATED SKIN.
Cutaneous Sensitivity.
NETRI has established an in vitro platform using hiPSC-derived sensory neurons suitable to assess the efficacy of compounds of interest in dermocosmetic such as anti-itching agents, pain management. In parallel NETRI is developing an innervated epidermis platform (coculture of neurons and keratinocytes) which is suitable to assess cold sensation, "well-being" effect, neuroinflammation (atopic dermatitis…), wound healing.
• ATP effect quantification for pruritus pathway..
• Lactic acid quantification for acidic pathway study.
• WS12 quantification for TRPM8 characterization.
• Temperature effect.
IRRIGATED SKIN.
Endothelial Cells & Keratinocytes.
NETRI has established an in vitro platform of keratinocytes cocultured with endothelial cells (HDMEC). The design of this plateform allows the communication between two cell types located in two compartments separated by a nanoporous membrane. This platform is suitable to assess compounds acting on the vascular component of the skin, to culture 3D tissue, to control shear stress, to link several chips and build a multi-organ-on-chip.
• Keratinocytes differenciation with/without HDMEC.
• HDMEC morphology with/without Keratinocytes.
OXIDATED STRESS LEVEL.
Anti-Oxidative Stress.
NETRI has validated the compatibility of an anti-oxidative stress assay on keratinocytes in NETRI's organs-on-chip platform, using AOP assay. In parallel, assays on sensory neurons with/without keratinocytes is under development.
• Anti-oxidant effect of Quercetin quantification.
INNERVATED MUSCLE.
NeuroMuscular Junction.
NETRI has successfully established an in vitro NeuroMuscular Junction (NMJ) platform using hiPSC-derived motor neurons and myotubes in a microfluidic device compatible with MEA.
This proof-of-concept enables functional analysis of healthy and diseased donors, ensuring more physiologically relevant models to support neuromuscular disease research.
• Bottox applications.
• Rejuvenation
OTHER POSSIBILITIES.
Next Applications & Models.
NETRI's patented technologies make it possible to innervate any organ, using electrophysiological recordings as a unique readout.
• Co-culture of hiPSC-derived sensory neurons, primary keratinocytes and endothelial cells (innervated and vascularized skin platform).
• Innervated biopsy or reconstructed skin culture (3D skin platform).
• Recreate skin conditions such as atopic dermatitis, psoriasis, wound.
• Specific organs ECM (liver, skin, brain, oncology) with BioMIMESYS®.
THE WAY WE WORK.
Out-Licensing.
• Co-development to set up new model & digital library.
• Technology transfert to integrate our technologies in your routine drug development.
• Out-licensing to feed your digital library with new signatures.
Sprint Test.
• Test the effets of your drug candidate in our lab to predict clincal outcomes rapidely.
• 6 or 8 weeks.
• PNS Neurons with Sensory & Motor Neurons and CNS Neurons with Glutamatergic & GABAergic Neurons.
Organs-on-chip devices.
• Ready-to-use organs on chip devices.
• 2D and 3D cell culture.
• Innervation & vascularization.
• Classic readouts (sequencing, dosing, imaging...).
• Electrophysiology readouts (MEA).
