Tri-culture & continious connectivity With the TriaLink™ microfluidic chip, the microchannels and NeoBento™ technologies are combined in a single microfluidic chip, to provide an unmatched user experience combined with the ability to culture up to 3 different cell types. Tri-culture Continuous connectivity by microchannels grooves technology 3 compartments for independant 3 different cell type cultures 8 or 16 microfluidic chips per NeoBento™ GET A QUOTE GET A SAMPLE READOUTS APPLICATIONS TECHNOLOGIES RESOURCES • Human cells • Rodent cells • Immunofluorescence • Live Dead Assays • Live staining • Liquid chromatography • Mass Spectoscopy • Lysis cells/supernatant analysis • ELISA • Calcium Imaging • Culture up to 3 different cell populations (neurons/glial cells or neurons/skin cells…) • Cell migration/chemotaxis (microglia cells for example) • Stress effect on skin cells (ROS…) • Neuroinflammation (Multiple sclerosis, Cerebral tumors…) • Microchannels grooves: Microchannels grooves rectangular slits engraved within the PDMS layer. Due to their micrometric measurements (3µm height, 5µm width), only cell extensions like for example neurites can grow within the microchannels, leaving the cell bodies within the compartments themselves. They allow also an enhanced axonal projection rate and continuous connectivity. • NeoBento™: The NeoBento™ format makes NETRI’s microfluidic devices as versatile and easy to handle as a typical 96-well plate, by being in the standard AINSI format. The NeoBento™ is compatible with routine control magnification imaging (x10, x20,…) and confocal or high magnification (x63 with water immersion) imaging. Its versatility allows users to perform High Throughput Screening, histochemical analysis and individual cell fixation. Finally, its compounds permit an optimized oxygenation without expensive equipment (pump or stirrer) and a controlled humidity of cell culture. Publications Microchannel patterning strategies for in vitro structural connectivity modulation of neural networks Electrokinetic confinement of axonal growth for dynamically configurable neural networks Microfluidic neurite guidance to study structure-function relationships in topologically complex population-based neural networks Microfluidic Devices Protocols NeuroFluidics™ – TriaLink Cells Types Protocols Sensory Neurons – Axol Bioscience Application Notes Evaluation of amyloid beta oligomers (AβO) effects on functional network integrity of rodent hippocampal neurons Posters Microfluidic high-throughput screening platform to screen pre-clinical stage compound effects on neurite outgrowth of human Motor Neurons post-injury Standardization criteria of hiPSC-derived neurons for Brain-on-Chip applications Modeling the human Brain-on-Chip with human iPSC-derived Glutamatergic neurons Standardization criteria of hiPSC-derived neurons for Brain-on-Chip applications
Growth kinetics With the DuaLink™ Delta Ultra microfluidic chip, the microchannels and NeoBento™ technologies are combined into a single microfluidic chip, to provide an unmatched user experience combined with an enhanced growth kinetics. Enhanced growth kinetics Triangular shape with microchannels of different length Continuous connectivity by microchannels grooves technology 3 compartments: 1 for cell culture, 1 for fluidic isolation and 1 to monitor axonal growth 8 or 16 microfluidic chips per NeoBento™ GET A QUOTE GET A SAMPLE READOUTS APPLICATIONS TECHNOLOGIES RESOURCES • Human cells (apparently healthy, diseased, engineered…) • Rodent cells • Immunofluorescence • Live Dead Assays • Live staining • Liquid chromatography • Mass Spectoscopy • Lysis cells/supernatant analysis • ELISA • Calcium Imaging • Growth kinetics quantification • Axonal regeneration visualization and quantification • Co-culture (in the same compartment, like Schwann cells/sensory neurons or astrocyte cells/motor neurons…) • Embryology • Axotomy • Peripherical pain • Motor neuron diseases (Amyotrophic Lateral Sclerosis, Spinal muscular atrophy…) • Neuroinflammation (Multiple sclerosis, Cerebral tumors…) • Microchannels tunnels: Microchannels tunnels are rectangular slits engraved within microfluidic chips. Due to their micrometric measurements (3µm height, 5µm width), only cell extensions like for example neurites can grow within the microchannels, leaving the cell bodies within the compartments themselves. They allow also an enhanced fluidic isolation and discontinuous connectivity. • NeoBento™: The NeoBento™ format makes NETRI’s microfluidic devices as versatile and easy to handle as a typical 96-well plate, by being in the standard AINSI format. The NeoBento™ is compatible with routine control magnification imaging (x10, x20,…) and confocal or high magnification (x63 with water immersion) imaging. Its versatility allows users to perform High Throughput Screening, histochemical analysis and individual cell fixation. Finally, its compounds permit an optimized oxygenation without expensive equipment (pump or stirrer) and a controlled humidity of cell culture. Publications Neurite growth kinetics regulation through hydrostatic pressure in a novel triangle-shaped neurofluidic system. Microchannel patterning strategies for in vitro structural connectivity modulation of neural networks Electrokinetic confinement of axonal growth for dynamically configurable neural networks Microfluidic neurite guidance to study structure-function relationships in topologically complex population-based neural networks Microfluidic Devices Protocols NeuroFluidics™ – DuaLink Delta Ultra Cells Types Protocols Sensory Neurons – Axol Bioscience Application Protocols Simple quantitative method of neurite length measurements in triangular microfluidic device. Application Notes Evaluation of amyloid beta oligomers (AβO) effects on functional network integrity of rodent hippocampal neurons Posters Neurite growth kinetics regulation through hydrostatic pressure in a novel triangle-shaped neurofluidic system. Modeling the human Brain-on-Chip with human iPSC-derived Glutamatergic neurons Standardization criteria of hiPSC-derived neurons for Brain-on-Chip applications Microfluidic high-throughput screening platform to screen pre-clinical stage compound effects on neurite outgrowth of human Motor Neurons post-injury High throughput standardised microfluidic platform for internalization and axonal transport explorations of a preclinical neurological compound
Co-culture & enhanced axonal projection rate With the DuaLink Ultra microfluidic chip, the microchannels and NeoBento™ technologies are combined in a single microfluidic chip, to provide an unmatched user experience combined with an enhanced axonal projection rate. Co-culture of different cell types Enhanced axonal projection and time to results Continuous connectivity by microchannels grooves technology 3 compartments : 2 for cell culture & 1 for fluidic isolation 8 or 16 microfluidic chips per NeoBento™ GET A QUOTE GET A SAMPLE READOUTS APPLICATIONS TECHNOLOGIES RESOURCES • Human cells • Rodent cells • Immunofluorescence • Live Dead Assays • Live staining • Liquid chromatography • Mass Spectoscopy • Lysis cells/supernatant analysis • ELISA • Calcium Imaging • Co-culture (neurons/skin cells, neurons/glial cells…) • Drug screening • Quantitative assays • Axonal transport • Skin nociception • Itch • Ageing • Wound healing • Toxicology • Virology (viral transfection in one compartment only) • Neuroinflammation (Multiple sclerosis, Cerebral tumors…) • Innervated skin • Neuromuscular junction • Motor neuron diseases (Amyotrophic Lateral Sclerosis…) • Microchannels grooves: Microchannels grooves rectangular slits engraved within the PDMS layer. Due to their micrometric measurements (3µm height, 5µm width), only cell extensions like for example neurites can grow within the microchannels, leaving the cell bodies within the compartments themselves. They allow also an enhanced axonal projection rate and continuous connectivity. • NeoBento™: The NeoBento™ format makes NETRI’s microfluidic devices as versatile and easy to handle as a typical 96-well plate, by being in the standard AINSI format. The NeoBento™ is compatible with routine control magnification imaging (x10, x20,…) and confocal or high magnification (x63 with water immersion) imaging. Its versatility allows users to perform High Throughput Screening, histochemical analysis and individual cell fixation. Finally, its compounds permit an optimized oxygenation without expensive equipment (pump or stirrer) and a controlled humidity of cell culture. Publications Microchannel patterning strategies for in vitro structural connectivity modulation of neural networks Electrokinetic confinement of axonal growth for dynamically configurable neural networks Microfluidic neurite guidance to study structure-function relationships in topologically complex population-based neural networks Microfluidic Devices Protocols NeuroFluidics™ – DuaLink Ultra Cells Types Protocols Sensory Neurons – Axol Bioscience Application Notes Evaluation of amyloid beta oligomers (AβO) effects on functional network integrity of rodent hippocampal neurons Posters Microfluidic high-throughput screening platform to screen pre-clinical stage compound effects on neurite outgrowth of human Motor Neurons post-injury Standardization criteria of hiPSC-derived neurons for Brain-on-Chip applications Modeling the human Brain-on-Chip with human iPSC-derived Glutamatergic neurons Standardization criteria of hiPSC-derived neurons for Brain-on-Chip applications