Vers une révolution de la recherche pharmaceutique
September 7, 2021
NETRI présente ses technologies de cerveau sur puce à la délégation officielle dans le cadre du salon Global Industrie
NETRI News.
NETRI PRÉSENTÉ DANS LA GAZETTE DU LABORATOIRE
March 22, 2021
La Gazette du Laboratoire consacre un article à Netri dans son n°273 de Mars 2021.
NETRI mis en avant dans Les Echos
June 29, 2020
L’article paru dans Les Echos met en avant NETRI et ses projets de développement.
Neurite growth kinetics regulation through hydrostatic pressure in a novel triangle-shaped neurofluidic system
May 6, 2020
Abstract Microfluidic neuro-engineering design rules have been widely explored to create in vitro neural networks with the objective to replicate physiologically relevant structures of the brain. Several neurofluidic strategies have been reported to study the connectivity of neurons, either within a population or between two separated populations, through the control of the directionality of their neuronal projections. Yet, the in vitro regulation of the growth kinetics of those projections remains challenging. Here, we describe a new neurofluidic chip with a triangular design that allows the accurate monitoring of neurite growth kinetics in a neuronal culture. This device permits to measure the maximum achievable length of projecting neurites over time and to report variations in neurite length under several conditions. Our results show that, by applying positive or negative hydrostatic pressure to primary rat hippocampal neurons, neurite growth kinetics can be tuned. This work presents a pioneering approach for the precise characterization of neurite length dynamics within an in vitro minimalistic environment.
Microchannel patterning strategies for in vitro structural connectivity modulation of neural networks
May 5, 2020
Abstract Compartmentalized microfluidic chips have demonstrated tremendous potential to create in vitro minimalistic environments for the reproduction of the neural circuitry of the brain. Although the protocol for seeding neural soma in these devices is well known and has been widely used in myriad studies, the accurate control of the number of neurites passing through the microchannels remains challenging. However, the regulation of axonal density among different groups of neurons is still a requirement to assess the inherent structural connectivity between neuronal populations. In this work, we report the effect of microchannel patterning strategies on the modulation of neuronal connectivity by applying dimensional modifications on microchannel-connected microfluidic chambers. Our results show that those strategies can modulate the direction and the number of neuronal projections of passage, therefore regulating the strength of the structural connections between two populations of neurons. With this approach, we provide innovative microfluidic design rules for the engineering of in vitro physiologically relevant neural networks.
NETRI received the Global Health & Pharma’s 2020 Biotechnology Awards
April 15, 2020
NETRI is honored to receive the Global Health Pharma’s Biotechnology Award 2020.
