Co-culture & enhanced axonal projection rate

With the DuaLink MEA microfluidic chip, the microchannels, NeoBento™ and MEA compatibility technologies are combined in a single microfluidic chip, to provide an unmatched user experience combined with an functional co-culture recording.

  • • Electrophysiology
  • • Human cells
  • • Rodent cells
  • • Immunofluorescence
  • • Live Dead Assays
  • • Live staining
  • • Liquid chromatography
  • • Mass Spectoscopy
  • • Lysis cells/supernatant analysis
  • • ELISA
  • • Calcium Imaging
  • • Co-culture (neurons/skin cells, neurons/glial cells...)
  • • Study of the functional activity of neurons
  • • Analysis of the functional influence of a non-neuronal cell population on neurons
  • • Drug screening
  • • Quantitative assays
  • • Axonal transport
  • • Skin nociception
  • • Itch
  • • Ageing
  • • Wound healing
  • • Toxicology
  • • Virology (viral transfection in one compartment only)
  • • Neuroinflammation (Multiple sclerosis, Cerebral tumors...)
  • • Innervated skin
  • • Neuromuscular junction
  • • Motor neuron diseases (Amyotrophic Lateral Sclerosis...)
Microchannels tunnels: Microchannels tunnels are rectangular slits engraved within microfluidic chips. Due to their micrometric measurements (3µm height, 5µm width), only cell extensions like for example neurites can grow within the microchannels, leaving the cell bodies within the compartments themselves. They allow also an enhanced fluidic isolation and discontinuous connectivity.
NeoBento™: The NeoBento™ format makes NETRI’s microfluidic devices as versatile and easy to handle as a typical 96-well plate, by being in the standard AINSI format. The NeoBento™ is compatible with routine control magnification imaging (x10, x20,...) and confocal or high magnification (x63 with water immersion) imaging. Its versatility allows users to perform High Throughput Screening, histochemical analysis and individual cell fixation. Finally, its compounds permit an optimized oxygenation without expensive equipment (pump or stirrer) and a controlled humidity of cell culture.
MEA Compatibility: Abiding by the 96-well microplate standard format, NeoBento™ MEA is a versatile container that enables electrophysiological recording of microfluidic experimentation while being compatible with standard equipment for liquid handling and imaging. DuaLink MEA allows to compare directly on the same chip the functional activity of 2 different neuronal types or the functional influence of a non-neuronal cell types on neurons.