Functional tri-culture recording

With the TriaLink MEA microfluidic chip, the microchannels, NeoBento™  and MEA compatibility technologies are combined in a single microfluidic chip, to provide an unmatched user experience combined with a discontinuous connectivity and neuronal functional analysis.

  • • Electrophysiology
  • • Human cells
  • • Rodent cells
  • • Immunofluorescence
  • • Live Dead Assays
  • • Live staining
  • • Liquid chromatography
  • • Mass Spectoscopy
  • • Lysis cells/supernatant analysis
  • • ELISA
  • • Calcium Imaging
  • • Culture up to 3 different cell populations (neurons/glial cells or neurons/skin cells...)
  • • Study of the functional activity of neurons
  • • Analysis of the functional influence of a non-neuronal cell population (astrocyte cells for example) on neurons
  • • Cell migration/chemotaxis (microglia cells for example)
  • • Stress effect on skin cells (ROS...)
  • • Neuroinflammation (Multiple sclerosis, Cerebral tumors...)
Microchannels tunnels: Microchannels tunnels are rectangular slits engraved within microfluidic chips. Due to their micrometric measurements (3µm height, 5µm width), only cell extensions like for example neurites can grow within the microchannels, leaving the cell bodies within the compartments themselves. They allow also an enhanced fluidic isolation and discontinuous connectivity.
NeoBento™: The NeoBento™ format makes NETRI’s microfluidic devices as versatile and easy to handle as a typical 96-well plate, by being in the standard AINSI format. The NeoBento™ is compatible with routine control magnification imaging (x10, x20,...) and confocal or high magnification (x63 with water immersion) imaging. Its versatility allows users to perform High Throughput Screening, histochemical analysis and individual cell fixation. Finally, its compounds permit an optimized oxygenation without expensive equipment (pump or stirrer) and a controlled humidity of cell culture.
MEA Compatibility: The bounding of NETRI microfluidic chips on MEA plates allows functional analysis combine with structural analysis. DuaLink MEA allows to compare directly on the same chip the functional activity of 2 different neuronal types or the functional influence of a non-neuronal cell types on neurons.