2-Cell Culture MEA Electrophysiology NEUROFLUIDICS™ LINE
Functional synaptic recording
With the DuaLink™ Shift MEA microfluidic chip, the microchannels, NeoBento™ and MEA compatibility technologies are combined in a single microfluidic chip, to provide an unmatched user experience combined with physiological synaptic compartmentalization and neuronal functional analysis.
- Functional synapse recording in the middle channel
- Asymmetrical shape with microchannels of different length (shorter length between the first and second channel than between the second and third channel)
- Discontinious connectivity
- 3 compartments crowded on MEA plate: 2 for cell culture & 1 for synaptic isolation
- 8 or 16 microfluidic chips per NeoBento™
- • Electrophysiology
- • Human cells
- • Rodent cells
- • Immunofluorescence
- • Live Dead Assays
- • Live staining
- • Liquid chromatography
- • Mass Spectoscopy
- • Lysis cells/supernatant analysis
- • ELISA
- • Calcium Imaging
- • Analysis of the functional influence of a non-neuronal cell population on synapse
- • Synaptic compartmentalization (pre-, post- and synaptic compartments)
- • Synaptic transmission and localization
- • Axonal transport
- • Mitochondrial transport
- • Microglial cells migration
- • Culture up to 2 different cell populations
- • Neuroinflammation
- • Huntington's disorders (cortico-striatal)
- • Study of the functional activity of neurons
- • Drug screening
- • Quantitative assays
• Microchannels tunnels: Microchannels tunnels are rectangular slits engraved within microfluidic chips. Due to their micrometric measurements (3µm height, 5µm width), only cell extensions like for example neurites can grow within the microchannels, leaving the cell bodies within the compartments themselves. They allow also an enhanced fluidic isolation and discontinuous connectivity.
• NeoBento™: The NeoBento™ format makes NETRI’s microfluidic devices as versatile and easy to handle as a typical 96-well plate, by being in the standard AINSI format. The NeoBento™ is compatible with routine control magnification imaging (x10, x20,...) and confocal or high magnification (x63 with water immersion) imaging. Its versatility allows users to perform High Throughput Screening, histochemical analysis and individual cell fixation. Finally, its compounds permit an optimized oxygenation without expensive equipment (pump or stirrer) and a controlled humidity of cell culture.
• MEA Compatibility: The bounding of NETRI microfluidic chips on MEA plates allows functional analysis combine with structural analysis. DuaLink MEA allows to compare directly on the same chip the functional activity of 2 different neuronal types or the functional influence of a non-neuronal cell types on neurons.
• NeoBento™: The NeoBento™ format makes NETRI’s microfluidic devices as versatile and easy to handle as a typical 96-well plate, by being in the standard AINSI format. The NeoBento™ is compatible with routine control magnification imaging (x10, x20,...) and confocal or high magnification (x63 with water immersion) imaging. Its versatility allows users to perform High Throughput Screening, histochemical analysis and individual cell fixation. Finally, its compounds permit an optimized oxygenation without expensive equipment (pump or stirrer) and a controlled humidity of cell culture.
• MEA Compatibility: The bounding of NETRI microfluidic chips on MEA plates allows functional analysis combine with structural analysis. DuaLink MEA allows to compare directly on the same chip the functional activity of 2 different neuronal types or the functional influence of a non-neuronal cell types on neurons.
Publications
Microchannel patterning strategies for in vitro structural connectivity modulation of neural networks
Electrokinetic confinement of axonal growth for dynamically configurable neural networks
Microfluidic neurite guidance to study structure-function relationships in topologically complex population-based neural networks
Microfluidic Devices Protocols
NeuroFluidics™ – DuaLink - DuaLink MEA
Cells Types Protocols
Sensory Neurons – Axol Bioscience
Application Notes
Evaluation of amyloid beta oligomers (AβO) effects on functional network integrity of rodent hippocampal neurons
Posters
Microfluidic high-throughput screening platform to screen pre-clinical stage compound effects on neurite outgrowth of human Motor Neurons post-injury
Standardization criteria of hiPSC-derived neurons for Brain-on-Chip applications
Modeling the human Brain-on-Chip with human iPSC-derived Glutamatergic neurons
Microchannel patterning strategies for in vitro structural connectivity modulation of neural networks
Electrokinetic confinement of axonal growth for dynamically configurable neural networks
Microfluidic neurite guidance to study structure-function relationships in topologically complex population-based neural networks
Microfluidic Devices Protocols
NeuroFluidics™ – DuaLink - DuaLink MEA
Cells Types Protocols
Sensory Neurons – Axol Bioscience
Application Notes
Evaluation of amyloid beta oligomers (AβO) effects on functional network integrity of rodent hippocampal neurons
Posters
Microfluidic high-throughput screening platform to screen pre-clinical stage compound effects on neurite outgrowth of human Motor Neurons post-injury
Standardization criteria of hiPSC-derived neurons for Brain-on-Chip applications
Modeling the human Brain-on-Chip with human iPSC-derived Glutamatergic neurons