Nerve Injury.

The Challenge.

Peripheral nerves are made of motor and sensory nerves, two very distinct types of neurons that are linked but each have their specific function. Accidents, surgeries and compressions (tumor, carpial canal syndrome) are examples of situations where traumatic injury to the spinal cord or nerves can induce neuropathic pain in addition to the debilitating motor and sensory symptoms. Two main axes of treatments have an important impact on patients’ quality of life: (i) regenerative medicine to reconstruct and regenerate nerve (ii) efficient pain relief. Conventional in vitro models are unable to isolate somas and neurites from neurons, thus limiting the discovery of regenerative therapies.

The Solution.

Organs-on-Chip enable the reproduction of highly segregated neural circuits, isolating neuron somas from their axons to mimic human neuroanatomical architecture for more relevant injury or treatment paradigms. This translational nerve injury platform enables the discovery of regenerative therapies. The microchannel technology used enhances axonal projections, providing an improved environment for neurotrophic or neurodegenerative readouts. The microfluidic isolation and architecture of our devices offer the advantage of targeting only neurites and not cell bodies as in conventional in vitro models.

DuaLink Shift NeuroFluidics Devices & PNS NeuroFluidics Cultures
hiPSC-derived sensory neurons somas in channel 1 & projections in channel 3
Nocadazole or Staurosporine in channel 2
Localized neurite injury with a reproducible protocol & neurite regeneration post-injury

Using organs-on-chip to model motor neurons injury.

Mirroring current in vivo models, such as nerve crush injury or nerve ligation which aim to mimic human nerve trauma, we have created a reproducible and standardized injury, cutting, motor and sensory axons only using a short and targeted detergent application. This platform enabled us to compare axonal regeneration after treatment with a neurotrophic molecule or a drug inhibiting neurite growth.

Microtubule destabilization by nocodazole as an internal neurodegenerative control
Enhancing neurite outgrowth by staurosporine, a broad-spectrum protein kinase inhibitor, used as an internal neurotrophic control

Screen your compound in our already industrial digital library with different combinatory responses and/or doses for IND/MAA submission.

Using organs-on-chip to model sensory neurons injury.

Mirroring current in vivo models, such as nerve crush injury or nerve ligation which aim to mimic human nerve trauma, we have created a reproducible and standardized injury, cutting, motor and sensory axons only using a short and targeted detergent application. This platform enabled us to compare axonal regeneration after treatment with a neurotrophic molecule or a drug inhibiting neurite growth..

Nocodazole more potent on axoCells™ sensory neurons vs. iCell motor neurons
Staurosporine less effective on iCell motor neurons, at the same concentration
Detection of differences in efficacy depending on the cell model used

Screen your compound in our already industrial digital library with different combinatory responses and/or doses for IND/MAA submission.

Benefits.

Relevant in vitro model​

Humanized in vitro traumatic injury models to recreate human anatomical architecture.
Human motor and sensory neurons models
Discovery of regenerative therapeutics
Enhance axonal projections

High-throughput & interoperable solutions

NeoBento™, the standard format for NeuroFluidics Devices chips, available up to 4 QuarterBentos™ (up to 16 chips).
Standard ANSI format (96-well plate)
Pump-free & expensive equipment-free
Standard equipment (liquid handling & imaging) compatibility

Compartmentalization & Fluidic isolation

Compartmentalized microfluidics devices enabling injury or treatment paradigms aligned with real life situations.
Target only neurites and not cell bodies
Improve environment for neurotrophic readouts
Mimic human nerve trauma

Readouts compatibility

In-depth reading of the data to better understand the study results and potential implications.
Electrophysiological recording (MEA)
Imaging (Immunofluorescence, Calcium Imaging…) .
Biochimic analysis (ELISA, Lysis cells analysis, Liquid Chromatography…)

Our Offers.

NETRI Services.

Technological Transfert
FTE & Screening Services
Co-development
Analytical Services

NETRI Products.

NeuroFluidics Devices
NeuroFluidics Cultures
NeuroFluidics Digital
Training & Organs-on-chip Kits

Summary.

MICROFLUIDICS IS NOW JUST
1-CLICK AWAY WITH NETRI SHOP

Discover our new exclusive package
organs-on-chip kits and all our
neuro-organs-on-chip devices.

EXCLUSIVE PACKAGE

ORGANS-ON-CHIP KITS

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