DuaLink Shift.
Features.
• Up to 16 data points
• 2 compartments for independant culture per chips
• 1 compartment for synapses isolation
• Microchannels technology of different lenghts to monitor growth kinetics & create synaptic compartment
Each chips are compartmentalized by microchannels technology. This enebles the creation of axodendritic synapses in the 2nd channel and the monitoring of axonal growth kinetics. Microchhanels technology enables also co-culture of different cell types (GABAergic neurons & glutamatergic neurons…). By adding the MEA option, record the electrical activity of synapses, isolated per compartment.
Products Details.
MEA-recording.
Specially designed to monitor the functional activity of synapses.
• 16 microfluidic chips per NeoBento™ MEA PRO with 672 electrodes
• 8 microfluidic chips per NeoBento™ MEA EDGE with 336 electrodes
• Synapses electrophysology activity isolation per compartment & remote stimulation
µTunnels.
Specially designed to recreate synapses isolation and monitor axonal growth kinetics.
• 16 microfluidic chips per NeoBento™ FULL
• 8 microfluidic chips per NeoBento™ LIGHT
• Discontinious connectivity
• Asymmetrical shape with microchannels of different length (shorter length between the first and second channel than between the second and third channel).
Benefits.
High-Throughput Format
NeoBento™, the standard format for NeuroFluidics Devices chips, available up to 4 QuarterBentos™ (up to 16 chips).
• Standard ANSI format (96-well plate)
• Pump-free & expensive equipment-free
• Standard equipment (liquid handling & imaging) compatibility
Compartmentalization
Compartmentalized microfluidics devices to co-culture 2 different cell types.
• Each compartment has its own media & coating solutions
• Discrimination of mode and mechanism of action
• Synaptic transmission & localization
Readouts Compatibility
In-depth reading of the data to better understand the study results and potential implications.
• Electrophysiological recording (MEA)
• Imaging (Immunofluorescence, Calcium Imaging…)
• Biochimic analysis (ELISA, Lysis cells analysis, Liquid Chromatography…)
Innervation of any Organ
Microchannels technology allow synaptic compartmentalization (pre-, post- and synaptic compartments).
• Neuroinflammation
• Inflammatory Bowel Disease
• Huntington’s disorders
Get Started Today.
NeuroFluidics MEA-recording Devices.
• 3 architectures : DuaLink MEA, DuaLink Shift MEA & TriaLink MEA
• 8 or 16 data points per plate
• Training & Organs-on-chip Kits
• Electrical neurons activity recording
NeuroFluidics Devices.
• 3 architectures : DuaLink, DuaLink Shift & TriaLink
• 8 or 16 data points per plate
• Training & Organs-on-chip Kits
• Imaging & Biochimic analyses readouts
Related Informations.
• Maisonneuve, B. G. C., Vieira, J., Larramendy, F., & Honegger, T. (2021). Microchannel patterning strategies for in vitro structural connectivity modulation of neural networks. BioRxiv, 2021-03. https://doi.org/10.1101/2021.03.05.434080
• Castiglione, H., Vigneron, P. A., Baquerre, C., Yates, F., Rontard, J., & Honegger, T. (2022). Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications. Pharmaceutics, 14(11), 2301. https://doi.org/10.3390/pharmaceutics14112301
• Gabriel-Segard, T.; Rontard, J.; Miny, L.; Dubuisson, L.; Batut, A.; Debis, D.; Gleyzes, M.; François, F.; Larramendy, F.; Soriano, A.; et al. (2023). Proof-of-Concept Human Organ-on-Chip Study: First Step of Platform to Assess Neuro-Immunological Communication Involved in Inflammatory Bowel Diseases. Int. J. Mol. Sci., 24, 10568. https://doi.org/10.3390/ijms241310568
• Rontard J, Maisonneuve BGC, Honegger T. (2023). Expanding human-based predictive models capabilities using organs-on-chip: A standardized framework to transfer and co-culture human iPSCs into microfluidic devices. Arch Pharm Pharma Sci. ; 7: 017-021. https://doi.org/10.29328/journal.apps.1001039
• Fuchs, Q., Batut, A., Gleyzes, M., Rontard, J., Miny, L., Libralato, M., Vieira, J., Debis, D., Larramendy, F., Honegger, T., Messe, M., Pierrevelcin, M., Lhermitte, B., Dontenwill, M., Entz-Werlé, N. (2021). Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions. J. Vis. Exp. (177), e62748, https://doi.org/10.3791/62748
• [2023] Chemotherapy-Induced Peripheral Neuropathy-On-Chip Model : Utilizing the strength of compartmentalization
• [2023] Compartimentalized culture of primary or hiPSC-derived neurons using an MEA-capable high-throughput organs-on-chip platform
• [2023] Surface tension-based cell seeding in NETRI microfluidic devices
• [2022] The DuaLink Chips how to improve reproducibility in compartmentalized co-cultures
• [2021] Evaluation of amyloid beta oligomers (AβO) effects on functional network integrity of rodent hippocampal neurons
• DuaLink Shift Protocol
• DuaLink Shift MEA Protocol
• Fixation & Immunostaining Protocol
• Recombinant adeno-associated virus Protocol
• Nerve Injury
• CNS
