CNS.
Features.
• DuaLink, DuaLink MEA or DuaLink Shift NeuroFluidics Devices
• Duplex Well NeuroFluidics Devices
• hiPSC Neurons / Rodent Neurons / Organoid
• Microchannels technology to segregate mode of action of compound and co-culture different cell types
CNS Models enable the creation of an in vitro compartmentalized model mimicking human anatomy. This enables also to co-culture neuronal and non-neuronal cell types (Neurons & Tumor Cells, Neurons & Immune Cells…). By adding the MEA option, evaluate the functional impact of compound on neurons.
Products Details.
Compartmentalized GABAergic & Glutamatergic Neurons.
Co-culture of hiPSC-derived glutamatergic neurons and GABAergic neurons for Central Nervous System applications.
• DuaLink or DuaLink MEA for screening of drug candidate
• DuaLink Shift MEA for synapses electrophysology activity isolation per compartment
• Electrophysiology, Imaging and Biochimis analysis readouts
Compartmentalized Glutamatergic & Glutamatergic Neurons.
Co-culture of hiPSC-derived glutamatergic neurons with non-neuronal cells (tumor, immune…) for oncology and immunology applications.
• DuaLink MEA for screening of drug candidate
• DuaLink Shift MEA for synapses electrophysology activity isolation per compartment
• Electrophysiology, Imaging and Biochimis analysis readouts
Compartmentalized Cortical & Hippocampal Neurons.
Co-culture of rodent cortical & hippocampal neurons for neurological dosorders applications.
•
DuaLink MEA to evaluate the functional impact of compound on neurons and for screening of drug candidate
• Electrophysiology, Imaging and Biochimis analysis readouts
Perfused Cerebral Organoid.
Culture of cerebral organoid up to 60 days in vitro for preclinical applications.
• Duplex Well to perfuse cerebral organoid
• Standard protocol for growing organoids-on-chip
• Characterization of brain organoid-on-chip
Benefits.
High-throughput & interoperable solutions
NeoBento™, the standard format for NeuroFluidics Devices chips, available up to 4 QuarterBentos™ (up to 16 chips).
• Standard ANSI format (96-well plate)
• Pump-free & expensive equipment-free
• Standard equipment (liquid handling & imaging) compatibility
Relevant in vitro model
Humanized in vitro CNS models for a wide variety of applications.
• Mimick human anatomy
• Up to 2 separated but connected neurons cell type
• Screening of potential drugs or therapeutic interventions for their effectiveness in preventing or treating therapies
Readouts compatibility
In-depth reading of the data to better understand the study results and potential implications.
• Electrophysiological recording (MEA)
• Imaging (Immunofluorescence, Calcium Imaging…)
• Biochimic analysis (ELISA, Lysis cells analysis, Liquid Chromatography…)
Compartmentalization & Fluidic isolation
Compartmented microfluidic devices for applying compounds specifically to a cell compartment.
• Discrimination of mode of action and mechanism of pathology
• Segregation of therapeutic modality: topical versus systemic
Get Started Today.
NeuroFluidics Services.
• Technological Transfert
• FTE & Screening Services
• Co-development
• Analytical Services
NeuroFluidics Devices.
• 3 architectures with or without MEA-recording
• 8 or 16 data points per plate
• Training
• Organs-on-chip Kits
Related Informations.
• Maisonneuve, B. G. C., Vieira, J., Larramendy, F., & Honegger, T. (2021). Microchannel patterning strategies for in vitro structural connectivity modulation of neural networks. BioRxiv, 2021-03. https://doi.org/10.1101/2021.03.05.434080
• Castiglione, H., Vigneron, P. A., Baquerre, C., Yates, F., Rontard, J., & Honegger, T. (2022). Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications. Pharmaceutics, 14(11), 2301. https://doi.org/10.3390/pharmaceutics14112301
• Gabriel-Segard, T.; Rontard, J.; Miny, L.; Dubuisson, L.; Batut, A.; Debis, D.; Gleyzes, M.; François, F.; Larramendy, F.; Soriano, A.; et al. (2023). Proof-of-Concept Human Organ-on-Chip Study: First Step of Platform to Assess Neuro-Immunological Communication Involved in Inflammatory Bowel Diseases. Int. J. Mol. Sci., 24, 10568. https://doi.org/10.3390/ijms241310568
• Rontard J, Maisonneuve BGC, Honegger T. (2023). Expanding human-based predictive models capabilities using organs-on-chip: A standardized framework to transfer and co-culture human iPSCs into microfluidic devices. Arch Pharm Pharma Sci. ; 7: 017-021. https://doi.org/10.29328/journal.apps.1001039
• Fuchs, Q., Batut, A., Gleyzes, M., Rontard, J., Miny, L., Libralato, M., Vieira, J., Debis, D., Larramendy, F., Honegger, T., Messe, M., Pierrevelcin, M., Lhermitte, B., Dontenwill, M., Entz-Werlé, N. (2021). Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions. J. Vis. Exp. (177), e62748, https://doi.org/10.3791/62748
• [2023] Compartimentalized culture of primary or hiPSC-derived neurons using an MEA-capable high-throughput organs-on-chip platform
• [2022] The DuaLink Chips how to improve reproducibility in compartmentalized co-cultures
• DuaLink Protocol
• DuaLink MEA Protocol
• DuaLink Shift Protocol
• Sensory Neurons Protocol
• Sensory Neurons MEA Protocol
• Fixation & Immunostaining Protocol
• Inflammatory Bowel Disease
• Alzheimer
• DuaLink MEA
• DuaLink Shift
