DuaLink, DuaLink MEA or DuaLink Shift NeuroFluidics Devices
Duplex Well NeuroFluidics Devices
hiPSC Neurons / Rodent Neurons / Organoid
Microchannels technology to segregate mode of action of compound and co-culture different cell types

CNS Models enable the creation of an in vitro compartmentalized model mimicking human anatomy. This enables also to co-culture neuronal and non-neuronal cell types (Neurons & Tumor Cells, Neurons & Immune Cells…). By adding the MEA option, evaluate the functional impact of compound on neurons.

Products Details.

Compartmentalized GABAergic & Glutamatergic Neurons.

Co-culture of hiPSC-derived glutamatergic neurons and GABAergic neurons for Central Nervous System applications.

DuaLink or DuaLink MEA for screening of drug candidate
DuaLink Shift MEA for synapses electrophysology activity isolation per compartment
Electrophysiology, Imaging and Biochimis analysis readouts

Compartmentalized Glutamatergic & Glutamatergic Neurons.

Co-culture of hiPSC-derived glutamatergic neurons with non-neuronal cells (tumor, immune…) for oncology and immunology applications.

DuaLink MEA for screening of drug candidate
DuaLink Shift MEA for synapses electrophysology activity isolation per compartment
Electrophysiology, Imaging and Biochimis analysis readouts

Compartmentalized Cortical & Hippocampal Neurons.

Co-culture of rodent cortical & hippocampal neurons for neurological dosorders applications.

​ DuaLink MEA to evaluate the functional impact of compound on neurons and for screening of drug candidate
Electrophysiology, Imaging and Biochimis analysis readouts

Perfused Cerebral Organoid.

Culture of cerebral organoid up to 60 days in vitro for preclinical applications.​

​Duplex Well to perfuse cerebral organoid
Standard protocol for growing organoids-on-chip
Characterization of brain organoid-on-chip


High-throughput & interoperable solutions

NeoBento™, the standard format for NeuroFluidics Devices chips, available up to 4 QuarterBentos™ (up to 16 chips).
Standard ANSI format (96-well plate)
Pump-free & expensive equipment-free
Standard equipment (liquid handling & imaging) compatibility

Relevant in vitro model

Humanized in vitro CNS models for a wide variety of applications.
Mimick human anatomy
Up to 2 separated but connected neurons cell type
Screening of potential drugs or therapeutic interventions for their effectiveness in preventing or treating therapies​

Readouts compatibility​

In-depth reading of the data to better understand the study results and potential implications.
Electrophysiological recording (MEA)
Imaging (Immunofluorescence, Calcium Imaging…)
Biochimic analysis (ELISA, Lysis cells analysis, Liquid Chromatography…)

Compartmentalization & Fluidic isolation​

Compartmented microfluidic devices for applying compounds specifically to a cell compartment.
Discrimination of mode of action and mechanism of pathology
Segregation of therapeutic modality: topical versus systemic

Get Started Today.

NeuroFluidics Services.

Technological Transfert
FTE & Screening Services
Analytical Services

NeuroFluidics Devices.

3 architectures with or without MEA-recording
​8 or 16 data points per plate
Organs-on-chip Kits

Related Informations.

Guichard, A., Remoué, N., & Honegger, T. (2022). In vitro sensitive skin models: review of the standard methods and introduction to a new disruptive technology. Cosmetics, 9(4), 67.
Maisonneuve, B. G. C., Vieira, J., Larramendy, F., & Honegger, T. (2021). Microchannel patterning strategies for in vitro structural connectivity modulation of neural networks. BioRxiv, 2021-03.
Castiglione, H., Vigneron, P. A., Baquerre, C., Yates, F., Rontard, J., & Honegger, T. (2022). Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications. Pharmaceutics, 14(11), 2301.​
Gabriel-Segard, T.; Rontard, J.; Miny, L.; Dubuisson, L.; Batut, A.; Debis, D.; Gleyzes, M.; François, F.; Larramendy, F.; Soriano, A.; et al. (2023). Proof-of-Concept Human Organ-on-Chip Study: First Step of Platform to Assess Neuro-Immunological Communication Involved in Inflammatory Bowel Diseases. Int. J. Mol. Sci., 24, 10568.
Rontard J, Maisonneuve BGC, Honegger T. (2023). Expanding human-based predictive models capabilities using organs-on-chip: A standardized framework to transfer and co-culture human iPSCs into microfluidic devices. Arch Pharm Pharma Sci. ; 7: 017-021.
Fuchs, Q., Batut, A., Gleyzes, M., Rontard, J., Miny, L., Libralato, M., Vieira, J., Debis, D., Larramendy, F., Honegger, T., Messe, M., Pierrevelcin, M., Lhermitte, B., Dontenwill, M., Entz-Werlé, N. (2021). Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions. J. Vis. Exp. (177), e62748,
[2023] Compartimentalized culture of primary or hiPSC-derived neurons using an MEA-capable high-throughput organs-on-chip platform
[2022] The DuaLink Chips how to improve reproducibility in compartmentalized co-cultures​
DuaLink Protocol
DuaLink MEA Protocol
DuaLink Shift Protocol
Sensory Neurons Protocol
Sensory Neurons MEA Protocol
Fixation & Immunostaining Protocol
Pediatric Glioblastoma
Inflammatory Bowel Disease
DuaLink MEA
DuaLink Shift


Discover our new exclusive package
organs-on-chip kits and all our
neuro-organs-on-chip devices.



Quickly and easily adopt organs-on-chip

into users’ research