PNS.
Features.
• DuaLink, DuaLink MEA or DuaLink Shift NeuroFluidics Devices
• Somas in one compartment
• Extensions in one compartment
• Microchannels technology to segregate mode of action of compound and isolate distal axons from soma and proximal axons
PNS Models enable the creation of an in vitro model mimicking human neuroanatomical architecture, for more relevant injury or treatment paradigms. By adding the MEA option, evaluate the functional impact of compound on neurons.
Products Details.
Directional Sensory Neurons Projections.
Culture of hiPSC-derived sensory neurons for Peripheral Nervous System applications.
• DuaLink or DuaLink MEA for screening of drug candidate
• DuaLink Shift for growth kinetics monitoring
• Electrophysiology, Imaging and Biochimis analysis readouts
Directional Motor Neurons Projections.
Culture of hiPSC-derived motor neurons for Peripheral Nervous System applications.
• DuaLink Shift for growth kinetics monitoring
• Imaging and Biochimis analysis readouts
• Injury & axonal regrowth after trauma model
Benefits.
High-throughput & interoperable solutions
NeoBento™, the standard format for NeuroPlatforms chips, available up to 4 QuarterBentos™ (up to 16 chips).
• Standard ANSI format (96-well plate)
• Pump-free & expensive equipment-free
• Standard equipment (liquid handling & imaging) compatibility
Relevant in vitro model
Humanized in vitro PNS models for a wide variety of applications.
• Human motor and sensory neurons models
• Enhance axonal projections
• Screening of potential drugs or therapeutic interventions for their effectiveness in preventing or treating therapies
Readouts compatibility
In-depth reading of the data to better understand the study results and potential implications.
• Electrophysiological recording (MEA)
• Imaging (Immunofluorescence, Calcium Imaging…)
• Biochimic analysis (ELISA, Lysis cells analysis, Liquid Chromatography…)
Compartmentalization & Fluidic isolation
Compartmentalized microfluidics devices enabling injury or treatment paradigms aligned with real life situations.
• Target only neurites and not cell bodies
• Discrimination of mode of action and mechanism of neurotoxicity
• Segregation of therapeutic modality: topical versus systemic
Get Started Today.
NeuroFluidics Services.
• Technological Transfert
• FTE & Screening Services
• Co-development
• Analytical Services
NeuroFluidics Devices.
• 3 architectures with or without MEA-recording
• 8 or 16 data points per plate
• Training
• Organs-on-chip Kits
Related Informations.
• Maisonneuve, B. G. C., Vieira, J., Larramendy, F., & Honegger, T. (2021). Microchannel patterning strategies for in vitro structural connectivity modulation of neural networks. BioRxiv, 2021-03. https://doi.org/10.1101/2021.03.05.434080
• Castiglione, H., Vigneron, P. A., Baquerre, C., Yates, F., Rontard, J., & Honegger, T. (2022). Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications. Pharmaceutics, 14(11), 2301. https://doi.org/10.3390/pharmaceutics14112301
• Gabriel-Segard, T.; Rontard, J.; Miny, L.; Dubuisson, L.; Batut, A.; Debis, D.; Gleyzes, M.; François, F.; Larramendy, F.; Soriano, A.; et al. (2023). Proof-of-Concept Human Organ-on-Chip Study: First Step of Platform to Assess Neuro-Immunological Communication Involved in Inflammatory Bowel Diseases. Int. J. Mol. Sci., 24, 10568. https://doi.org/10.3390/ijms241310568
• Rontard J, Maisonneuve BGC, Honegger T. (2023). Expanding human-based predictive models capabilities using organs-on-chip: A standardized framework to transfer and co-culture human iPSCs into microfluidic devices. Arch Pharm Pharma Sci. ; 7: 017-021. https://doi.org/10.29328/journal.apps.1001039
• Fuchs, Q., Batut, A., Gleyzes, M., Rontard, J., Miny, L., Libralato, M., Vieira, J., Debis, D., Larramendy, F., Honegger, T., Messe, M., Pierrevelcin, M., Lhermitte, B., Dontenwill, M., Entz-Werlé, N. (2021). Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions. J. Vis. Exp. (177), e62748, https://doi.org/10.3791/62748
• [2023] Compartimentalized culture of primary or hiPSC-derived neurons using an MEA-capable high-throughput organs-on-chip platform
• [2022] The DuaLink Chips how to improve reproducibility in compartmentalized co-cultures
• DuaLink Protocol
• DuaLink MEA Protocol
• DuaLink Shift Protocol
• Sensory Neurons Protocol
• Sensory Neurons MEA Protocol
• Fixation & Immunostaining Protocol
• Nerve Injury
• DuaLink MEA
• DuaLink Shift
