DuaLink.
Features.
• Up to 16 data points
• 2 compartments for independant culture per chips
• 1 compartment for fluidic isolation
• Microchannels technology to compartmentalize culture and connect channels
Each chips are compartmentalized by microchanels technology. This enables co-culture of different cell types (keratinocytes & neurons, neurons & intestinal cells…). Microchhanels technology enables also innervation of the 3rd channel by neuron endings (innervated skin, innervated gut…). By adding the MEA option, record the electrical activity of neurons to decode data from any innervated organ.
Products Details.
MEA-Recording.
Specially designed to monitor the functional activity of 2 physiological compartments of cell populations, compatible with Maestro PRO or EDGE ( Axion BioSystems).
• 16 microfluidic chips per NeoBento™ MEA PRO with 672 electrodes
• 8 microfluidic chips per NeoBento™ MEA EDGE with 336 electrodes
• Cell type electrophysology activity isolation per compartment & remote stimulation
µTunnels.
Specially designed to recreate different cellular microenvironment for co-culture of 2 different cell types with fluidic isolation.
• 16 microfluidic chips per NeoBento™ FULL
• 8 microfluidic chips per NeoBento™ LIGHT
• Discontinious connectivity
µGrooves.
Specially designed to enhance axonal projection and recreate different cellular microenvironments for co-culture of 2 different cell types.
• 16 microfluidic chips per NeoBento™ FULL
• 8 microfluidic chips per NeoBento™ LIGHT
• Continuous connectivity
Benefits.
High-Throughput Format
NeoBento™, the standard format for NeuroPlatforms chips, available up to 4 QuarterBentos™ (up to 16 chips).
• Standard ANSI format (96-well plate)
• Pump-free & expensive equipment-free
• Standard equipment (liquid handling & imaging) compatibility
Compartmentalization
Compartmentalized microfluidics devices to co-culture 2 different cell types.
• Each compartment has its own media & coating solutions
• Discrimination of mode and mechanism of action
• Pathophysiological applications
Readouts Compatibility
In-depth reading of the data to better understand the study results and potential implications.
• Electrophysiological recording (MEA)
• Imaging (Immunofluorescence, Calcium Imaging…)
• Biochimic analysis (ELISA, Lysis cells analysis, Liquid Chromatography…)
Innervation of any Organ
Microchannels technology allow only the passage of neuron endings leaving cell bodies in compartment. .
• Innervated Skin
• Neuromuscular junction
• Innervated Gut
Get Started Today.
NeuroFluidics MEA-Recording Devices.
• 3 architectures : DuaLink MEA, DuaLink Shift MEA & TriaLink MEA
• 8 or 16 data points per plate
• Training & Organs-on-chip Kits
• Electrical neurons activity recording
NeuroFluidics Devices.
• 3 architectures : DuaLink, DuaLink Shift & TriaLink
• 8 or 16 data points per plate
• Training & Organs-on-chip Kits
• Imaging & Biochimic analyses readouts
Related Informations.
• Maisonneuve, B. G. C., Vieira, J., Larramendy, F., & Honegger, T. (2021). Microchannel patterning strategies for in vitro structural connectivity modulation of neural networks. BioRxiv, 2021-03. https://doi.org/10.1101/2021.03.05.434080
• Castiglione, H., Vigneron, P. A., Baquerre, C., Yates, F., Rontard, J., & Honegger, T. (2022). Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications. Pharmaceutics, 14(11), 2301. https://doi.org/10.3390/pharmaceutics14112301
• Gabriel-Segard, T.; Rontard, J.; Miny, L.; Dubuisson, L.; Batut, A.; Debis, D.; Gleyzes, M.; François, F.; Larramendy, F.; Soriano, A.; et al. (2023). Proof-of-Concept Human Organ-on-Chip Study: First Step of Platform to Assess Neuro-Immunological Communication Involved in Inflammatory Bowel Diseases. Int. J. Mol. Sci., 24, 10568. https://doi.org/10.3390/ijms241310568
• Rontard J, Maisonneuve BGC, Honegger T. (2023). Expanding human-based predictive models capabilities using organs-on-chip: A standardized framework to transfer and co-culture human iPSCs into microfluidic devices. Arch Pharm Pharma Sci. ; 7: 017-021. https://doi.org/10.29328/journal.apps.1001039
• Fuchs, Q., Batut, A., Gleyzes, M., Rontard, J., Miny, L., Libralato, M., Vieira, J., Debis, D., Larramendy, F., Honegger, T., Messe, M., Pierrevelcin, M., Lhermitte, B., Dontenwill, M., Entz-Werlé, N. (2021). Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions. J. Vis. Exp. (177), e62748, https://doi.org/10.3791/62748
• [2023] Chemotherapy-Induced Peripheral Neuropathy-On-Chip Model : Utilizing the strength of compartmentalization
• [2023] Compartimentalized culture of primary or hiPSC-derived neurons using an MEA-capable high-throughput organs-on-chip platform
• [2023] Surface tension-based cell seeding in NETRI microfluidic devices
• [2022] The DuaLink Chips how to improve reproducibility in compartmentalized co-cultures
• [2021] Evaluation of amyloid beta oligomers (AβO) effects on functional network integrity of rodent hippocampal neurons
• DuaLink Protocol
• DuaLink MEA Protocol
• Fixation & Immunostaining Protocol
• Recombinant adeno-associated virus Protocol
• Chemotherapy-Induced Peripheral Neuropathy
• Alzheimer Disease
• Innervated Skin 2D
• PNS
• CNS
