Skin.
Features.
• DuaLink or Duplex Well NeuroFluidics Devices
• Primary keratinocytes in one compartment
• hiPSC Sensory Neurons or endothelial cells in one compartment
• Microchannels technology to connect keratinocytes by endings sensory neurons
• Membrane technology to perfuse keratinocytes by endothelial cells
Skin Models enables the creation of an in vitro innervated or irrigated skin model. By adding the MEA option, evaluate the functional impact of ingredient or product on neurons.
Products Details.
Innervated Skin.
Co-culture of primary keratinocytes and hiPSC-derived sensory neurons.
• DuaLink or DuaLink MEA
• Electrophysiology, Imaging and Biochimis analysis readouts
• Fully human in vitro model
Irrigated Skin.
Co-culture of primary keratinocytes and endothelial cells.
• Duplex Well
• Imaging and Biochimis analysis readouts
• Fully human in vitro model
Benefits.
Cosmetic Regulation
Cosmetic regulation ensures product safety, labeling accuracy, and adherence to quality standards in the beauty industry.
• No animal testing
• Drug and cosmetic efficacy and toxicity
• Compatible with standard analytical method and tools
Compartmentalization
Compartmentalized microfluidics devices to recreate skin compartment and neurons or endothelial cells compartment.
• Discrimination of mode and mechanism of action
• Pathophysiological applications (sensitive skin and pruritus, inflammatory dermatosis…)
Fully Compatible
NeoBento™, the standard SBS format for NeuroPlatforms for readouts and lab equipments compatibility.
• Pump-free & expensive equipment-free
• Standard equipment compatibility
• Electrophysiology, imaging and biochimic analysis readouts compatibility
PDMS Optically Transparent
Soft silicon material, optically transparent for microscopy compatiblity and porous gas permability.
• Imaging (Immunofluorescence, Calcium Imaging…)
• Confocal Imaging
• Perfusion-free with high biocompatibility
Get Started Today.
NeuroFluidics Services.
• Technological Transfert
• FTE & Screening Services
• Co-development
• Analytical Services
NeuroFluidics Devices.
• 3 architectures with or without MEA-recording
• 8 or 16 data points per plate
• Training
• Organs-on-chip Kits
Related Informations.
• Maisonneuve, B. G. C., Vieira, J., Larramendy, F., & Honegger, T. (2021). Microchannel patterning strategies for in vitro structural connectivity modulation of neural networks. BioRxiv, 2021-03. https://doi.org/10.1101/2021.03.05.434080
• Castiglione, H., Vigneron, P. A., Baquerre, C., Yates, F., Rontard, J., & Honegger, T. (2022). Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications. Pharmaceutics, 14(11), 2301. https://doi.org/10.3390/pharmaceutics14112301
• Gabriel-Segard, T.; Rontard, J.; Miny, L.; Dubuisson, L.; Batut, A.; Debis, D.; Gleyzes, M.; François, F.; Larramendy, F.; Soriano, A.; et al. (2023). Proof-of-Concept Human Organ-on-Chip Study: First Step of Platform to Assess Neuro-Immunological Communication Involved in Inflammatory Bowel Diseases. Int. J. Mol. Sci., 24, 10568. https://doi.org/10.3390/ijms241310568
• Rontard J, Maisonneuve BGC, Honegger T. (2023). Expanding human-based predictive models capabilities using organs-on-chip: A standardized framework to transfer and co-culture human iPSCs into microfluidic devices. Arch Pharm Pharma Sci. ; 7: 017-021. https://doi.org/10.29328/journal.apps.1001039
• Fuchs, Q., Batut, A., Gleyzes, M., Rontard, J., Miny, L., Libralato, M., Vieira, J., Debis, D., Larramendy, F., Honegger, T., Messe, M., Pierrevelcin, M., Lhermitte, B., Dontenwill, M., Entz-Werlé, N. (2021). Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions. J. Vis. Exp. (177), e62748, https://doi.org/10.3791/62748
• [2023] Compartimentalized culture of primary or hiPSC-derived neurons using an MEA-capable high-throughput organs-on-chip platform
• [2022] The DuaLink Chips how to improve reproducibility in compartmentalized co-cultures
• [2021] Evaluation of amyloid beta oligomers (AβO) effects on functional network integrity of rodent hippocampal neurons
• DuaLink Protocol
• DuaLink MEA Protocol
• Sensory Neurons Protocol
• Sensory Neurons MEA Protocol
• Fixation & Immunostaining Protocol
• DuaLink MEA
